Streptomyces flavusporus sp. nov., a Novel Actinomycete Isolated from Naidong, Xizang (Tibet), China

The exploration of <i>Streptomyces</i> from extreme environments presents a particularly compelling avenue for novel compound discovery. A Gram-positive, pink-pigmented <i>Streptomyces</i> strain designated HC307^T was isolated from a soil sample collected in Xizang (Tibet), China. The exploration of <i>Streptomyces</i> from extreme environments presents a particularly compelling avenue for novel compound discovery. In this study, the 16S rRNA sequence of strain HC307^T exhibited the highest similarity with <i>Streptomyces prasinosporus</i> NRRL B-12431^T (97.5%) and <i>Streptomyces chromofuscus</i> DSM 40273^T (97.3%), which were below 98.7%. The draft genome of the bacteria was 10.0 Mb, with a G+C content of 70.0 mol%. The average nucleotide identity (ANI) values of strain HC307^T and similar type strains ranged from 78.3% to 87.5% (<95%). The digital DNA-DNA hybridization (dDDH) values ranged from 22.6% to 33.9% (<70%), which was consistent with the results obtained from phylogenetic tree analysis. Phenotypically, this bacterium grew within the temperature range of 25-40 °C, at a pH range of 5 to 9, and in NaCl concentrations from 0% to 6% (<i>w</i>/<i>v</i>). The polar lipid profile of strain HC307^T was diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified lipids. The analysis of 32 biosynthetic gene clusters (BGCs) indicated the strain's capacity to synthesize diverse compounds. Phylogenetic and phenotypic analyses demonstrated that strain HC307^T represented a novel species within the genus <i>Streptomyces</i>, and proposed the name <i>Streptomyces flavusporus</i> sp. nov., with strain HC307^T (=DSM 35222^T=CGMCC 32047^T). The strain was deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen and the China General Microbiological Culture Collection Center for patent procedures under the Budapest Treaty.