Synthesis and Application of Novel Benzothiazole Fluorescent Probes

As a new detection method, fluorescent probe plays a very important role in many fields. The variety of probe is more and more, the specificity of detection is more and more strong, the range is more and more wide, the detection sensitivity is more and more good. Compared with the traditional detection method, the fluorescence probe has higher selectivity and no obvious toxic and negative effect, and has good sensitivity and simple and convenient operation, can be very good for living cells or living animals, plants and other advantages of tracking imaging, Therefore, the development of new fluorescent probes has now become the focus of scientific research. In many fluorescence probes, the small molecule fluorescence probe has definite element composition and molecular weight, and has many advantages in synthesis, analytical performance, reproducibility and biological imaging. This article using ethanol as solvent, 2-aminophenol and 5-methylsalicylaldehyde as starting materials. A fluorescence probe targeting 2-(benzo [ D ] thiazole-2-yl) -6-(hydrazone methyl) -4-methylphenol (BTH-MPH) was designed and synthesized. Its structure was characterized by hydrogen spectrum, carbon spectrum and mass spectrum analysis. The absorption spectrum of the probe was measured by fluorescence photometer, and the cytotoxicity of the probe was measured by CCK-8 method, and the imaging of the cells was detected by confocal laser microscopy. The results show the target probe has the correct structure, pH range 3-8, and strong absorption peak at 510 and 570 nm. The cell survival rate was above 92.34% after 24 hours incubation at 7 concentrations in the range of 0-50 μm, the target probe can be used to detect living cells.