Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4

With the continuous development and application of CRISPR-based resistance removal technologies, CRISPR-Cas9, Cas12f1, and Cas3 have gradually come into focus. However, it remains uncertain which system exhibits more potent efficacy in the removal of bacterial resistance. This study verifies that CRISPR-Cas9, Cas12f1, and Cas3 can eradicate the carbapenem-resistant genes KPC-2 and IMP-4 and restore the sensitivity of drug-resistant model bacteria to antibiotics. Among the three CRISPR systems, the CRISPR-Cas3 system showed the highest eradication efficiency. Although each system has its advantages and characteristics, our results provide guidance on the selection of the CRISPR system from the perspective of resistance gene removal efficiency, contributing to the further application of CRISPR-based bacterial resistance removal technologies.

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Publication Details

Journal
Microbiology Spectrum
Published
2025-04-28
DOI
https://doi.org/10.1128/spectrum.02572-24
Citations
4
Primary Topic
CRISPR and Genetic Engineering
Type
article
Field-Weighted Citation Impact
3.22

Funders

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article

Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4

Kaiyang Ding, Jun Huang, Hongbin Song, Luyao Huang et al.
4 citations
Microbiology Spectrum
CRISPR and Genetic Engineering
3.22
article

Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4

Kaiyang Ding, Jun Huang, Hongbin Song, Luyao Huang, Hai‐Feng Pan, Ligui Wang, Jiahui Chen, Chao Wang, Jiao Fan, Xinying Du, Shaofu Qiu, Hongbo Liu, Zhengquan Yuan
article en
4 citations

Abstract

With the continuous development and application of CRISPR-based resistance removal technologies, CRISPR-Cas9, Cas12f1, and Cas3 have gradually come into focus. However, it remains uncertain which system exhibits more potent efficacy in the removal of bacterial resistance. This study verifies that CRISPR-Cas9, Cas12f1, and Cas3 can eradicate the carbapenem-resistant genes KPC-2 and IMP-4 and restore the sensitivity of drug-resistant model bacteria to antibiotics. Among the three CRISPR systems, the CRISPR-Cas3 system showed the highest eradication efficiency. Although each system has its advantages and characteristics, our results provide guidance on the selection of the CRISPR system from the perspective of resistance gene removal efficiency, contributing to the further application of CRISPR-based bacterial resistance removal technologies.

Microbiology SpectrumVol. 13(6)
Capital Medical University (CN), Chinese Center For Disease Control and Prevention (CN), Anhui Medical University (CN), Taiwan Centers for Disease Control (TW), China Medical University (CN)
National Natural Science Foundation of China, National Science and Technology Major Project
Good health and well-being
Openalex Percentile: Top 9%
CRISPR and Genetic Engineering
3.22
DOIOpen Access PDFOpenAlex
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