Automated fluorine-18 radiolabeling via an alkyne–azide cycloaddition reaction on a dual peptide-functionalized liposome surface for in vivo PET imaging
The results demonstrate a higher tumor/background ratio, a faster clearance rate, and a lower uptake in healthy brain tissue and peripheral regions for mApoE- and MSLP-functionalized liposomes than for non-functionalized liposomes, prompting further characterization. On the contrary, radiolabeled liposome uptake is higher in the majority of peripheral organs for non-functionalized liposomes. Hence, fluorine-18-labeled liposomes can be reliably used for in vivo PET tracking of multifunctionalized nanoparticles, enabling effective investigation of their potential as drug delivery systems.
Authors
- Paolo Rainone (ORCID: https://orcid.org/0000-0001-8893-8797)
- Antonia I. Antoniou (ORCID: https://orcid.org/0000-0001-8579-2999)
- Marco Iannone (ORCID: https://orcid.org/0000-0002-1170-5570)
- Sara Pellegrino (ORCID: https://orcid.org/0000-0002-2325-3583)
- Arianna Amenta (ORCID: https://orcid.org/0000-0001-9111-9768)
- Rosa Maria Moresco (ORCID: https://orcid.org/0000-0002-2771-9387)
- Silvia Valtorta (ORCID: https://orcid.org/0000-0003-2871-6046)
- Marcelo Kravicz (ORCID: https://orcid.org/0000-0002-0341-2516)
- Francesca Re (ORCID: https://orcid.org/0000-0003-1374-567X)
- Daniele Passarella (ORCID: https://orcid.org/0000-0001-6180-9581)
- Sergio Todde (ORCID: https://orcid.org/0000-0001-8913-0538)
- Pierfausto Seneci (ORCID: https://orcid.org/0000-0001-9709-7344)
- Stefano Stucchi
- Elisa Vino
- E Turolla
Institutions
- Vita-Salute San Raffaele University (IT)
- University of Milan (IT)
- Institute of Molecular Bioimaging and Physiology (IT)
- University of Milano-Bicocca (IT)
Publication Details
- Journal
- Frontiers in Pharmacology
- Published
- 2025-04-28
- DOI
- https://doi.org/10.3389/fphar.2025.1566257
- Citations
- 1
- Primary Topic
- Click Chemistry and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- European Commission
- Università degli Studi di Milano-Bicocca
- Ministero dell’Istruzione, dell’Università e della Ricerca
- Regione Lombardia
- Fondazione Regionale per la Ricerca Biomedica