DNA metabarcoding unveils authenticity and adulteration in commercial Chinese polyherbal preparations: Renshen Jianpi Wan as a critical case study
This study establishes a systematic framework for molecular authentication of complex herbal formulations, providing technical support for reliable identification of botanical drugs. While DNA metabarcoding offers valuable insights into CCPP composition, authentication of heavily processed ingredients remains a significant technical limitation. The integration with complementary analytical methods such as metabolomics could provide more comprehensive quality assessment in future studies, demonstrating the necessity of multi-analytical approaches in ensuring the authenticity of traditional medicine.
Authors
- Fuquan Long (ORCID: https://orcid.org/0000-0001-7355-503X)
- Shilin Zhou (ORCID: https://orcid.org/0000-0001-6158-1193)
- Yanmei Zhang (ORCID: https://orcid.org/0000-0001-7142-5469)
- Junmei Niu (ORCID: https://orcid.org/0000-0002-9772-7032)
- Jing Zhou (ORCID: https://orcid.org/0000-0002-0252-2588)
- Weixian Li (ORCID: https://orcid.org/0009-0009-5302-8617)
- Ting Zhou (ORCID: https://orcid.org/0000-0002-1880-5371)
- Zhenwen Liu (ORCID: https://orcid.org/0000-0001-9397-2260)
- Bingqian Yang
- Yiyi Weitu
Institutions
- Kunming Medical University (CN)
- Second Affiliated Hospital of Kunming Medical College (CN)
- Yunnan Academy of Forestry (CN)
Publication Details
- Journal
- Frontiers in Pharmacology
- Published
- 2025-04-28
- DOI
- https://doi.org/10.3389/fphar.2025.1584065
- Citations
- 1
- Primary Topic
- Environmental DNA in Biodiversity Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.72
Funders
- National Natural Science Foundation of China
- Yunnan Provincial Science and Technology Department