The contrasting roles of fungal and bacterial diversity and composition in shaping the multifunctionality of rhizosphere and bulk soils across large-scale bamboo forests

Soil microbes regulate nutrient cycling, organic matter decomposition, and other processes, thereby maintaining soil multifunctionality (SMF). However, the relationship between microbial characteristics and soil multifunctionality has primarily been studied in bulk soils, with less attention to rhizosphere soils. Moreover, this relationship remains unclear within a single forest type across large scales. In this study, we selected six sites across the distribution range of moso bamboo (Phyllostachys edulis (Carrière) J. Houz.) in China to quantify the relationship between microbial communities and soil multifunctionality in both rhizosphere and bulk soil, and to evaluate how abiotic factors influence this relationship. Our results showed that microbial diversity was negatively correlated with SMF, while the key microbial drivers (bacteria or fungi) of SMF varied between soil compartments (i.e., rhizosphere and bulk soil). Soil variables influenced SMF in bulk soils by affecting bacterial diversity and fungal composition, whereas in rhizosphere soils, soil variables influenced SMF primarily by affecting fungal diversity and composition, suggesting that different characteristics of bacterial and fungal communities drive SMF. Climatic factors exert a more significant influence on the multifunctionality of rhizosphere soils compared to bulk soils. Considering the intricate interplay between plants and soil microbes, our study highlights the importance of integrating SMF and microbial community structure within distinct soil compartments.