Formulation And Characterization of Nanolipid Carriers Loaded With Eugenol And Glycyrrhizic Acid: An In Vitro Evaluation Of Their Antimicrobial Potential

Natural remedies are increasingly being used to cure and prevent infectious diseases, particularly those caused by multidrug-resistant (MDR) pathogens. These days, medication resistance is a worldwide issue. As a result, having medications on hand that can combat MDR infections is crucial. The spice clove (Syzygium aromaticum) is well-known for its many biological qualities. Eugenol is the main active ingredient in its essential oil (EO), which also contains other active compounds. However, eugenol's combined action with other components is responsible for the oil's biological effects. The purpose of this study is to examine how eugenol-glycyrrhizic acid extract nanolipid carriers (EGAE-NCL) can combat MDR bacteria that cause infections, illnesses, or problems in humans, including Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa. High shear force homogenization was used to create EGAE-NLC nanoparticles, which contained clove oil, licorice extract (glycyrrhizic acid), and clove extract (eugenol). SEM and HPLC were used to verify that EGAE-NLC nanoparticles were crystalline and pure. We concluded that the produced EGAE-NLC nanoparticles were crystalline in nature based on the SEM data. The average size of the EGAE-NLC nanoparticles was between 29 and 71 nm, and they demonstrated strong antibacterial activity. The research results demonstrated EGAE-NLC nanoparticles can inhibit the growth of MDR isolate S. aureus, P. aeruginosa and C. albicans.