Microscopic and Molecular Identification of <i>Plasmodium falciparum</i> in Patients of Wasit Province, Iraq

The study created electron transfer PCR, using self-quenching primers to identify several species of Plasmodium. The potential use of polymerase chain reaction (PCR) for the molecular identification of malaria parasites in Wasit, Iraq. QIAGEN extracted DNA from desiccated blood spots. PCR analysis was conducted twice on each of the 200 samples. Positive samples had a CT value of 60 or below. Fifty samples were evaluated for accuracy using nested PCR. A TaqMan-based real-time PCR technique was used on these samples. PCR successfully detected 20 positive samples from a total of 200. The samples tested positive by both nested and TaqMan methodologies. Neither PCR nor TaqMan successfully identified a positive sample in a selection of 50; however, nested PCR did. In conclusion, Nested PCR has more sensitivity than PET-PCR; nonetheless, it is not the optimal selection for high-throughput sample screening. Nested PCR serves as an effective alternative test for the quick screening of several samples in the laboratory due to its straightforward implementation and cost-effectiveness.