RESULTS OF ALZHEIMER’S DISEASE ANIMAL MODEL INDUCTION BASED ON THE INTRAHIPPOCAMPAL INJECTION OF AMYLOID Β-PEPTIDE (1-42) AT VIETNAM MILITARY MEDICAL UNIVERSITY

Objectives: To evaluate some behaviors and histological results of the hippocampus in a rat model of Alzheimer's disease (AD). Methods: A longitudinal, descriptive study was conducted on 21 Wistar rats. Intrahippocampal amyloid beta (Aβ) injection was performed, followed by behavioral testing and analysis and hippocampal histological examination. Results: Rats in the AD model showed learning impairment with a reduced average latency time (52.09 ± 10.67s) and reduced latency time on the 5th day (27.08 ± 6.88s) during the learning phase of the Morris water maze (MWM) test. Memory impairment was indicated by a decreased percentage of spontaneous alternations (34.45 ± 7.03%) in the Y-maze test, reduced time spent (14.84 ± 4.61s), and shorter distance traveled (2.26 ± 0.82m) in the target quadrant during the probe trial of the MWM test. Neurodegeneration in the hippocampus was observed, with an increased degeneration score (1.83 ± 0.31). Conclusion: Intrahippocampal Aβ injection is an effective method for inducing the AD model in rats, characterized by learning and memory impairments as well as neurodegeneration in experimental animals.