Evaluation of biological activity via biospeckle laser imaging

We describe the statistical characteristics of optical speckle patterns formed by illuminating biological tissues, commonly called biospeckles. The predominant techniques used to gather information from the movement of speckle patterns are detailed. Using vegetable tissues, we monitored the senescence process and created vascularization maps of leaf tissues. The Fujii method, which has been modified, has emerged as the most effective approach for highlighting the biological activity across leaf tissues. This technique relies on the presence of fluid flow to create highly detailed maps of tissue microcirculation. The method of temporal contrast evaluation produced a significant spectral activity map, which allowed for the detection of both instant and invisible bruised tissue. The evaluation revealed that biological specimens can exhibit a unique time history of speckle pattern (THSP) patterns, which may serve as a biological signature for the sample. Additionally, an activity index was calculated to define the assay's activity under different biological conditions, and the results were tested and verified across multiple samples.