Generation of a gene-corrected isogenic human iPS cell line (CSUASOi006-A-2) from a retinitis pigmentosa patient using CRISPR/Cas9 technology
Retinitis pigmentosa (RP) is a heterogeneous group of hereditary eye disorders characterized by a progressive degeneration of the light-sensing photoreceptor cells in the retina. Currently, there are no effective treatments. In a previous study, we generated a human induced pluripotent stem (iPS) cell line (CSUASOi006-A) from an RP patient carrying a PRPF8 (c.C5792T) mutation. In this study, we corrected the c.5792C > T mutation in the PRPF8 gene using CRISPR/Cas9 technology and generated an isogenic control cell line (CSUASOi006-A-2). This provides an important cellular resource for RP research.
Authors
- Xihao Sun (ORCID: https://orcid.org/0000-0002-3470-2006)
- Ruting Zhang (ORCID: https://orcid.org/0000-0001-9695-763X)
- Yuqin Liang (ORCID: https://orcid.org/0000-0002-2448-4746)
- Zekai Cui (ORCID: https://orcid.org/0000-0003-3670-6219)
- Jiansu Chen (ORCID: https://orcid.org/0000-0001-6703-9573)
- Yuexi Chen (ORCID: https://orcid.org/0000-0001-8720-5516)
- Yuan Liang (ORCID: https://orcid.org/0000-0002-9143-9670)
- Xiaoxue Li (ORCID: https://orcid.org/0000-0003-0004-9528)
- Hang Chen (ORCID: https://orcid.org/0000-0002-6356-3930)
- Wen‐Wei Li (ORCID: https://orcid.org/0000-0001-9280-0045)
- Jianing Gu (ORCID: https://orcid.org/0000-0003-2241-5533)
- Chengcheng Ding
- Chunwen Duan
Institutions
- Central South University (CN)
- Shandong Eye Hospital (CN)
- Xiangya Hospital Central South University (CN)
- First Affiliated Hospital of Jinan University (CN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-04-26
- DOI
- https://doi.org/10.1016/j.scr.2025.103727
- Primary Topic
- Retinal Development and Disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.00