In-vitro Regeneration Potential of Tinospora cordifolia (Wild.) Miers Using Nodal Explant Grown in Murashige and Skoog (MS) Medium

This study aims to analyze the effects of various shoot-promoting growth hormones on the micropropagation of above-ground plant parts of Tinospora cordifolia. Tinospora cordifolia is a valuable medicinal plant with diverse therapeutic properties, including immunomodulatory, anti-inflammatory, and antioxidant activities. The plant's extracts are in high demand for pharmaceutical applications, and micropropagation can ensure a consistent supply of high-quality plant material. The experiment will be conducted applying Murashige and Skoog (MS) media which is augmented with varying concentrations of: (BAP), Thidiazuron (TDZ) and Kinetin. Various parameters are recorded throughout the experiment, including the number of days to primordia initiation, shoot count, node count, shoot length, leaf count, and intensity of callus formation. Among the three hormones tested, BAP and kinetin exhibited a more favorable response compared to the other hormone in MS media.