Improving sustainable isopropanol production in engineered Escherichia coli W via oxygen limitation
This study identifies slightly microaerobic conditions (q<sub>O2</sub> ~ 10 mmol g<sup>- 1</sup> h<sup>- 1</sup>) as the currently best conditions for microbial isopropanol production on lactose and whey in E. coli W. In the future, optimizing the carbon flux around the pyruvate node, ensuring sufficient NADPH supply, and establishing a feedback control loop to control process variables affecting oxygen transfer to the culture, could make microbial isopropanol production feasible at an industrial scale.
Authors
- Regina Kutscha (ORCID: https://orcid.org/0000-0002-6720-7634)
- Stefan Pflügl (ORCID: https://orcid.org/0000-0001-8472-5073)
- Dominic Uhlir
Institutions
- University of Vienna (AT)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-04-26
- DOI
- https://doi.org/10.1186/s12934-025-02720-1
- Citations
- 1
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Technische Universität Wien
- Österreichische Forschungsförderungsgesellschaft
- Technische Universität Wien Bibliothek