High-Throughput Field Phenotyping Using Unmanned Aerial Vehicles (UAVs) for Rapid Estimation of Photosynthetic Traits

Efficient measurement of photosynthetic traits, such as the maximum carboxylation rate of Rubisco (Vcmax) and electron transport rate (Jmax), is essential for advancing research and breeding aimed at enhancing crop productivity. Traditional methods are time-intensive, which limits their scalability. Remote sensing presents an opportunity for estimating these traits; however, it often lacks an affordable platform for effective spatial mapping, a critical aspect of phenotyping. This study explored the use of unmanned aerial vehicle (UAV) multispectral data to estimate and spatially map photosynthetic traits in tea chrysanthemums during the branching and budding stages under an open canopy. Over six field experiments across varieties conducted in 2022-2023, we captured canopy reflectance using UAV-mounted multispectral sensors, calculated spectral indices, and measured the photosynthetic traits of the upper leaves using a portable photosynthesis system. The results indicated that certain indices, particularly those incorporating green and red-edge bands, effectively estimated photosynthetic traits, with the simplified canopy chlorophyll content index (SCCCI) yielding the most accurate Vcmax estimates (R² ​= ​0.52) and the chlorophyll vegetation index (CVI) providing the best estimates for Jmax (R² ​= ​0.38). The integration of variable selection with partial least squares regression (PLSR) modeling further enhanced the precision of the model (Vcmax: R² ​= ​0.70; Jmax: R² ​= ​0.63). Our findings demonstrate that UAV-acquired multispectral data can effectively map photosynthetic traits with high spatial resolution, establishing it as a valuable tool for rapid phenotyping and spatial assessment of photosynthetic capacity in crop fields.