Mitochondrial Sensitivity to Submaximal [ADP] Following Bed Rest: A Novel Two‐Phase Approach Associated With Fibre Types
A novel mathematical approach (two sequential hyperbolic functions) for the fitting of JO<sub>2</sub> versus [ADP] data obtained by high-resolution respirometry on permeabilized skeletal muscle fibres, obtained in humans and rats, provided an alternative fitting of the experimental data compared to the traditional Michaelis-Menten kinetics equation. This alternative model allowed the identification of two distinct phases in the responses, which were related to fibre type composition. A first phase, characterized by low apparent K<sub>m</sub> and V<sub>max</sub> values, was correlated with the percentage of less oxidative (Type 2A + 2X) MyHC isoforms. A second phase, characterized by high apparent K<sub>m</sub> and V<sub>max</sub>, was related to more oxidative (Type 1) MyHC isoforms.
Authors
- Elena Monti (ORCID: https://orcid.org/0000-0002-3767-0855)
- Benjamin D. Thackray
- Boštjan Šimunič (ORCID: https://orcid.org/0000-0003-1565-7833)
- Miriam Isola (ORCID: https://orcid.org/0000-0002-6391-1720)
- Bruno Grassi (ORCID: https://orcid.org/0000-0003-3188-1659)
- Andrew J. Murray (ORCID: https://orcid.org/0000-0002-0929-9315)
- Rado Pišot (ORCID: https://orcid.org/0000-0002-2508-7830)
- Giovanni Baldassarre (ORCID: https://orcid.org/0000-0002-4182-9216)
- Antonio Filippi (ORCID: https://orcid.org/0000-0003-4308-8746)
- Marco Narici (ORCID: https://orcid.org/0000-0003-0167-1845)
- Giovanna Lippe (ORCID: https://orcid.org/0000-0003-0042-5052)
- Lucrezia Zuccarelli (ORCID: https://orcid.org/0000-0003-2447-4684)
- Giuseppe Sirago (ORCID: https://orcid.org/0000-0001-7005-8708)
- Maria De Martino (ORCID: https://orcid.org/0000-0002-5322-1531)
- Alice E. Knapton
Institutions
- University of Udine (IT)
- University of Padua (IT)
- University of Cambridge (GB)
- PUH Team (Slovenia) (SI)
- University of Lausanne (CH)
Publication Details
- Journal
- Journal of Cachexia Sarcopenia and Muscle
- Published
- 2025-04-25
- DOI
- https://doi.org/10.1002/jcsm.13775
- Citations
- 2
- Primary Topic
- Spaceflight effects on biology
- Type
- article
- Field-Weighted Citation Impact
- 2.33
Funders
- British Heart Foundation
- Agenzia Spaziale Italiana
- Evelyn Trust
- Università degli Studi di Udine