Endothelial glycocalyx in different flow regions of the trabecular outflow pathway in bovine eyes

No regional differences were found between HF and LF regions, but glycocalyx thickness progressively increased from the proximal to the distal outflow pathway, potentially reflecting varying shear stress conditions. The significantly higher percentage of GVs with I-pores containing glycocalyx lining the inner cellular membrane compared to those without I-pores suggests a relationship between aqueous outflow dynamics and glycocalyx synthesis. These findings provide a morphological basis for future research on glycocalyx alterations in glaucoma and their impact on outflow resistance.