Abstract LB449: InTraSeq unveils transcriptomic and proteomic landscapes of Th17 differentiation

Abstract Single-cell RNA sequencing (scRNA-seq) has revolutionized our understanding of cellular heterogeneity. However, scRNA-seq primarily focuses on gene expression, limiting our ability to fully characterize cellular states and their underlying regulatory mechanisms. Here, we present InTraSeq, a novel multimodal assay that enables simultaneous measurement of mRNA, surface markers, cytoplasmic proteins, and nuclear proteins within individual cells. InTraSeq utilizes oligo-barcoded antibodies to profile protein expression in conjunction with scRNA-seq. We demonstrate the application of InTraSeq to study Th17 cell differentiation, revealing novel insights into the dynamics of this complex process. InTraSeq provides a powerful tool for investigating cellular heterogeneity and regulatory mechanisms, enabling a more comprehensive understanding of cellular function in health and disease. Citation Format: Majd Ariss. InTraSeq unveils transcriptomic and proteomic landscapes of Th17 differentiation [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_2):Abstract nr LB449.