Characterization and Biotechnology of Three New Strains of Basidial Fungi as Promising Sources of Biologically Active Substances

The study of new strains of basidiomycetes as sources of biologically active substances is a promising direction in modern biotechnology. This work aims to isolate new cultures of the fungi <i>Daedaleopsis tricolor</i>, <i>Pycnoporellus fulgens</i> and <i>Trichaptum abietinum</i> from natural fruiting bodies and to improve their growth conditions on solid nutrient media. The identification of fungi was performed based on their morphological features and using the Sanger sequencing method. Cultivation was carried out by placing inoculum in the middle of a Petri dish and at the edge, which provided a more comprehensive definition of the characteristics of colonies and fungus hyphae. New strains were registered in Genbank Overview. The optimal cultivation temperature was 27 °C for all studied strains. The highest radial growth was observed on synthetic medium for <i>D. tricolor</i> (5.26 mm/day) and <i>T. abietinum</i> (7.5 mm/day)<i>,</i> and on synthetic medium with lignin for <i>P. fulgens</i> (2.98 mm/day). The biomass amount of <i>D. tricolor</i> KS11 was 133.25 mg at 9 days of cultivation, that of <i>P. fulgens</i> KS12 was 86.73 mg at 16 days, and that of <i>T. abietinum</i> KS10 was 227.33 mg at 6 days. New strains of fungi can be used to obtain biologically active substances for the food, pharmaceutical and cosmetic industries.