Three-dimensional super-resolution imaging of whole cells using a high numerical aperture oblique-plane microscope
We present HOPE-STORM, a high-numerical-aperture oblique-plane microscope enabling whole-cell super-resolution imaging with an effective NA of 1.40. The system is compatible with DNA-PAINT, resolving nuclear pore complex (NPC) structures at 7.5 nm. We demonstrate dual-color super-resolution imaging of intact cells and, for the first time, to our knowledge, quantify the 3D organization of dynamin-related protein 1 (DRP1) complexes at mitochondrial fission sites. Our findings provide structural insights into DRP1 dynamics and mitochondrial fission mechanisms.
Authors
- Lingjia Gu (ORCID: https://orcid.org/0000-0002-4909-2263)
- Shihang Luo
- Lei Wang (ORCID: https://orcid.org/0000-0001-7977-5543)
- Wei Ji (ORCID: https://orcid.org/0000-0003-0303-0830)
- Jing Lü (ORCID: https://orcid.org/0000-0002-5791-4749)
- Tao Xu (ORCID: https://orcid.org/0000-0001-7544-0551)
- Chunyan Fan (ORCID: https://orcid.org/0000-0001-6673-6238)
- Xiaowen Zhao (ORCID: https://orcid.org/0000-0001-6224-8624)
- Weixing Li (ORCID: https://orcid.org/0000-0003-1589-1494)
- Shun Xiong (ORCID: https://orcid.org/0000-0002-8233-1609)
- Tianjie Yang
- Zhuoli Ding
Institutions
- University of Chinese Academy of Sciences (CN)
- Huazhong University of Science and Technology (CN)
Publication Details
- Journal
- Optics Letters
- Published
- 2025-04-25
- DOI
- https://doi.org/10.1364/ol.550216
- Citations
- 1
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.63
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- Beijing Municipal Natural Science Foundation
- National Key Research and Development Program of China