Mixed Mode Chromatographic Stationary Phases in Pharmaceutical Peptide Analysis

The mixtures of pharmaceutical peptides generally include peptide fragments and similar peptides. Reversed-phase HPLC (RP) is the most frequently utilized method for pharmaceutical peptide analysis. These stationary phases could not sometimes separate complicated peptide mixtures. Free silanols can additionally lead to trailing peaks that reduce peak resolution. These challenges leas to explore the advantages of mixed-mode stationary phase in peptide analysis. Particulate or monolithic mixed-mode stationary phases contains polar and hydrophobic groups. Polar groups are inserted into the alkyl chains “embedded” or used mask the residual silanols “Polar end-capping” . This review aimed to discuss the role of particulate and monolithic mixed-mode stationary phases in the analysis of pharmaceutical peptides. Detailed description of these phases were presented. Examples of peptide separation using these phases were shown.