Applications of Spectrophotometric Methods and SDS-PAGE In Quantification of Total Soluble Proteins in Escherichia coli From Raw Meat, Offals and Rectal Swabs of Cattle in Gwagwalada

The study was carried out to quantify the total soluble protein profile of nine Escherichia coli isolates from raw meat, offals and rectal swab by spectrotrophotmetric method. The results indicated that the virulent strains with highest concentration of protein from rectal swab was 19.19 µg followed by raw meat 15.88 µg while the lowest concentration of proteins from raw meat was 8.35 µg, lowest for offals was 10.55 µg and the lowest for rectal swab was 12.95 µg. this was followed by the electropherogram of the nine E. coli strains by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). E. coli isolates from raw meat, offals and rectal swab when compared with the marker, were similar in their molecular weights. The obtained results displayed bands that are both faint and dense, appearing at different approximate molecular weights of 17 KDa, 34 KDa, 43 KDa, and 55 KDa. The results of our protein profiles showed a clear indication of the genetic relatedness between diver’s members of the Escherichia strains. It is an indication of their close genetic relatedness within the members of the Enterobacteriaceae species. E. coli organisms are therefore regarded as heterogenous groups of bacteria of pathogenic significance which are responsible for virulence and pathopgenicity. This information is novel as it could be used as a standard for further classification and understanding the diversity of the members of the genus Escherichia. Further studies including the analysis of the virulent genes cloning and expression of genes of interest is necessary to identify new strains of Escherichia coli.