Investigation of The Bioethanol and Antioxidant Potential of Borodinellopsis texensis Grown in Wastewater under Various Copper Concentrations

Microalgae have the potential to grow at a rapid rate, which allows them to surpass other living creatures in terms of their prevalence in scientific research settings. Through their growth in wastewater, they are able to make a contribution to the treatment of wastewater. In addition, microalgae's metabolic components make them suitable for industrial applications. In this work, we investigated at how copper stress at various concentrations affected the biomass, carbohydrate, bioethanol, and antioxidant activities of Borodinellopsis texensis cultivated in 25% wastewater. The maximum biomass concentration was 0.79 ± 0.02 g/L at control group. In addition, carbohydrate content was the highest in a medium with 0.025 g/L copper, at 0.29 ± 0.02 g/L. The bioethanol productivities of Borodinellopsis texensis were 174.4 mg/g for 0.025 g/L Cu and 141.8 mg/ g for the control. DPPH, CAT, SOD and APX tests were performed to examine the antioxidant activities of Borodinellopsis texensis. The DPPH maximum scavenging, CAT, SOD, and APX activities of Borodinellopsis texensis were 83%, 17.4 µmol/mg, 8.87 µmol/mg, and 32.7 µmol/mg at 0.025 g/L of Cu, respectively. We are going to continue our investigation into the ways in which microalgae react when it is subjected to copper stress in larger reactors in the future with the assistance of this study.