Effect of salicylic acid and some plant extracts on growth and stimulation of cardiac glycosidic compounds of Digitalis lanata in vitro

The research aimed to investigate the effect of salicylic acid, and some plant extracts some factors on growth indicators and stimulating cardiac glycosides in digitalis leaves, in the Plant Tissue Culture Laboratory - College of Agricultural Engineering Sciences - University of Baghdad from 10-2-2022 to 6-1-2023. Branch cultures of the digitalis plant were obtained from planting the 1 cm length shoot tips, grown from sterilized seeds cultured in MS medium provided with 0.5 mg.L-1 of BA. After obtaining a sufficient number of vegetative branches by proliferating them more than once, they were cultured in MS medium provided with salicylic acid at the concentrations of 0, 1, 3, 5, and 7 mg.L-1, ginger extract at the concentrations of 0, 3, 5 and 7 g.L-1, and black pepper extract at the concentrations of 0, 3,5, and 7 g.L-1 as independent experiments. The results showed that salicylic acid concentration was superior 3mg.L-1 was significantly superior in the number of branches, number of leaves, fresh and dry weight, chlorophyll content, and the cardiac digoxin, reordering 2.67 branches.plant-1, 0.467cm, 9.67 leaves.plant-1, 1.328 g, 0.879 g., 1.376 mg, and 28.48 µg.g-1; and the treatment of ginger extract at a concentration of 5 gm l-1 and black pepper extract at a concentration of 3 gm l-1 was significantly superior separately in same traits, attaining 4.33 branches.plant-1, 14.43 leaves.plant-1, 1.567 g, 0.823 g, 1.497mg, and 29.32 µg.g-1 for ginger extract; and 2.133 branches.plant-1, 1,243 cm, 14.99 leaves.plant-1, 1.174 g, 0.659 g, 0.855 g.100 g-1fresh weight, and 29.66 µg.g-1 for black pepper extract, respectively.