Investigation of Trichinella spiralis protein extract on humoral and cellular immunity in mice

Relevance. The present experiment was performed on 113 CBA × C57BL/6 mice males. T. spiralis extract was administered to mice intraperitoneally once and six times at the dose level of 100 mkg/animal; Trichinella larvae were administered per os at the rate of 10 larvae per 1 g. The immunotropic effect was evaluated on the 8th and 21st days after a single and last six-fold administration of the extract. The effects on humoral immunity were determined in the hemagglutination reaction; cellular immunity was assessed in delayed-type hypersensitivity (DTH) reaction to sheep erythrocytes. In one variant of the experiment (on the 8th day after a single and last six-fold administration of the protein extract), a histological examination of samples of organs (spleen, thymus, lymph nodes, liver) involved in the functioning of the immune system was performed. Results. According to the results of comprehensive assessment of humoral and cellular immunity, using specific indices and microscopic analysis data, it can be concluded that Trichinella somatic extract showed immunosuppressive effect with the most pronounced effect on cellular immunity. It is possible that the suppression of cellular immune response is associated with cytotoxic effect of T. spiralis extract on cells, including those of immune system.