Functional-based parcellation of the mouse prefrontal cortex for network perturbation analysis
The prefrontal cortex (PFC) is a brain region involved in higher-order cognitive processes such as attention, emotional regulation, and social behavior. However, the delineation of distinct subdivisions within the mouse PFC and their contributions to the broader brain network function remain debated. This study utilizes resting-state functional magnetic resonance imaging (MRI) from a cohort of 100 C57BL/6J wild-type mice to derive the functional connectivity (FC)-based parcellation of the mouse PFC with voxel resolution. Our findings reveal clusters that deviate from the established anatomical subdivisions within the cingulate and prelimbic areas while aligning in infralimbic and orbital cortices. Upon the chemogenetic perturbation of one of the clusters, FC perturbations occur only within the functional network linked to the targeted cluster and do not spread to neighboring anatomical areas or functional clusters. We propose FC-based parcellation as a valuable approach for tracking the site of activation and network impact of neurostimulation strategies.
Authors
- Christina Grimm (ORCID: https://orcid.org/0000-0002-2379-4160)
- Marija Markicevic (ORCID: https://orcid.org/0000-0003-0983-432X)
- Iurii Savvateev (ORCID: https://orcid.org/0009-0000-5159-7239)
- Nicole Wenderoth (ORCID: https://orcid.org/0000-0002-3246-9386)
- Valerio Zerbi (ORCID: https://orcid.org/0000-0001-7984-9565)
- Rafael Polanía (ORCID: https://orcid.org/0000-0002-6176-6806)
- Alessandro Gozzi (ORCID: https://orcid.org/0000-0002-5731-4137)
- Joanes Grandjean (ORCID: https://orcid.org/0000-0001-8413-0491)
- David Sastre
Institutions
- University of Geneva (CH)
- Radboud University Nijmegen (NL)
- Radboud University Medical Center (NL)
- Italian Institute of Technology (IT)
- Yale University (US)
- ETH Zurich (CH)
- Center for Neuroscience and Cognitive Systems (IT)
- École Polytechnique Fédérale de Lausanne (CH)
Publication Details
- Journal
- Cell Reports
- Published
- 2025-04-25
- DOI
- https://doi.org/10.1016/j.celrep.2025.115622
- Citations
- 1
- Primary Topic
- Neural dynamics and brain function
- Type
- article
- Field-Weighted Citation Impact
- 1.43
Funders
- National Science Foundation
- European Commission
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Eidgenössische Technische Hochschule Zürich
- Horizon 2020 Framework Programme
- European Research Council
- Horizon 2020