A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger
In this study we have established an efficient system for the construction of CRISPR-Cpf1 vectors that are able to deliver a large number of gRNAs for multiplex genome editing in Aspergillus species. Our strategy allows multiple specific genomic modifications to be performed in a time frame of less than two weeks, and we envision this will be able to speed up cell factory construction efforts significantly.
Authors
- Abel Peter van Esch
- Bernd Gerhartz
- Fabiano Jares Contesini (ORCID: https://orcid.org/0000-0001-9436-1226)
- Uffe Hasbro Mortensen (ORCID: https://orcid.org/0000-0002-7794-7273)
- Kate E. Royle (ORCID: https://orcid.org/0000-0003-3501-8289)
- Samuel Mathew Maurice Prudence
Institutions
- Dairy Management (US)
- Technical University of Denmark (DK)
Publication Details
- Journal
- Fungal Biology and Biotechnology
- Published
- 2025-04-25
- DOI
- https://doi.org/10.1186/s40694-025-00196-7
- Citations
- 4
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 3.22