antiSMASH 8.0: extended gene cluster detection capabilities and analyses of chemistry, enzymology, and regulation
Microorganisms synthesize small bioactive compounds through their secondary or specialized metabolism. Those compounds play an important role in microbial interactions and soil health, but are also crucial for the development of pharmaceuticals or agrochemicals. Over the past decades, advancements in genome sequencing have enabled the identification of large numbers of biosynthetic gene clusters directly from microbial genomes. Since its inception in 2011, antiSMASH (https://antismash.secondarymetabolites.org/), has become the leading tool for detecting and characterizing these gene clusters in bacteria and fungi. This paper introduces version 8 of antiSMASH, which has increased the number of detectable cluster types from 81 to 101, and has improved analysis support for terpenoids and tailoring enzymes, as well as improvements in the analysis of modular enzymes like polyketide synthases and nonribosomal peptide synthetases. These modifications keep antiSMASH up-to-date with developments in the field and extend its overall predictive capabilities for natural product genome mining.
Authors
- Aleksandra Korenskaia (ORCID: https://orcid.org/0000-0003-3002-6458)
- Hannah E. Augustijn (ORCID: https://orcid.org/0000-0002-1862-6699)
- Valérie de Crécy‐Lagard (ORCID: https://orcid.org/0000-0002-9955-3785)
- Christophe Corre (ORCID: https://orcid.org/0000-0002-4292-9315)
- Kai Blin (ORCID: https://orcid.org/0000-0003-3764-6051)
- Barbara R. Terlouw (ORCID: https://orcid.org/0000-0002-4058-6718)
- Marnix H. Medema (ORCID: https://orcid.org/0000-0002-2191-2821)
- Friederike Biermann (ORCID: https://orcid.org/0000-0002-9152-2562)
- Simon J. Shaw (ORCID: https://orcid.org/0000-0002-0548-1881)
- Joleen Masschelein (ORCID: https://orcid.org/0000-0003-4366-3675)
- Nadine Ziemert (ORCID: https://orcid.org/0000-0002-7264-1857)
- Eric J. N. Helfrich (ORCID: https://orcid.org/0000-0001-8751-3279)
- Sam E. Williams
- David Meijer (ORCID: https://orcid.org/0000-0001-6406-4394)
- Justin J. J. van der Hooft (ORCID: https://orcid.org/0000-0002-9340-5511)
- Mitja M. Zdouc (ORCID: https://orcid.org/0000-0001-6534-6609)
- Marc G. Chevrette (ORCID: https://orcid.org/0000-0002-7209-0717)
- Lisa Vader (ORCID: https://orcid.org/0009-0008-5454-3306)
- Pablo Cruz‐Morales (ORCID: https://orcid.org/0000-0002-6751-8897)
- Tilmann Weber (ORCID: https://orcid.org/0000-0002-8260-5120)
- Zachary L. Reitz (ORCID: https://orcid.org/0000-0003-1964-8221)
- Gilles P. van Wezel (ORCID: https://orcid.org/0000-0003-0341-1561)
- José D D Cediel-Becerra (ORCID: https://orcid.org/0000-0001-6496-2071)
- Sopida Wongwas (ORCID: https://orcid.org/0000-0002-5770-9745)
- Robert A. Koetsier (ORCID: https://orcid.org/0000-0002-4477-5401)
- Judit Szenei
- Alejandro E Segurado Luchsinger (ORCID: https://orcid.org/0000-0002-2364-3143)
Institutions
- Goethe University Frankfurt (DE)
- Leiden University (NL)
- Florida Museum of Natural History (US)
- University of California, Santa Barbara (US)
- University of Johannesburg (ZA)
- Novo Nordisk Foundation (DK)
- Senckenberg - Leibniz Institution for Biodiversity and Earth System Research (DE)
- University of Florida (US)
- University of Warwick (GB)
- VIB-KU Leuven Center for Microbiology (BE)
- Czech Academy of Sciences, Institute of Microbiology (CZ)
- University of Tübingen (DE)
- Wageningen University & Research (NL)
- Technical University of Denmark (DK)
- KU Leuven (BE)
Publication Details
- Journal
- Nucleic Acids Research
- Published
- 2025-04-25
- DOI
- https://doi.org/10.1093/nar/gkaf334
- Citations
- 309
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 429.50
Funders
- Deutsches Zentrum für Infektionsforschung
- National Research Foundation
- Deutsche Forschungsgemeinschaft
- Danmarks Grundforskningsfond
- Fonds Wetenschappelijk Onderzoek
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Hessisches Ministerium für Wissenschaft und Kunst
- Novo Nordisk
- Novo Nordisk Fonden
- National Institutes of Health
- Horizon 2020 Framework Programme
- HORIZON EUROPE Framework Programme
- H2020 European Research Council