Optimization and Validation for Simultaneous Estimation of Metformin and Furosemide by Using RP-UFLC

Background Metformin is a Biguanide derivative used to treat the type-II diabetes, Furosemide is a loop diuretic used in the treatment of hypertension associated with edema. Aim: Our aim is to create an analytical method for the simultaneous measurement of Metformin and Furosemide by using RP-UFLC techniques.Materials and Methods In the present study, liquid chromatography with UV detector operating at 240 nm and a C-18 column was used. A gradient elution was performed using a mobile phase containing methanol and water containing 0.1% orthophosphoric acid in a ratio of 50:50 V/V and injected at a rate of 1 mL min-1.Results The developed method was validated according to the ICH guidelines. Linear response was detected over a concentration ranging from 400-2400 ngmL-1 for both drugs with R2=0.9982 for Metformin and R2=0.9989 for Furosemide.Conclusion The accuracy and precision of the method were within acceptable limit indicating its reliability for routine analysis.