Ligand‐Driven Annular‐Epitaxial Growth of CuS‐Au Heterostructures as Trinity Plasmonic Nanozyme for Multimode Diagnosis of Pathogenic Bacteria

This study presents a novel method to control the site-selective growth of Au nanostars on CuS nanodisc substrate, it indicates that the surfactant ligands play a key role in the architecture control, only CTAC and homologous series with appropriate affinity to CuS can direct the annular-epitaxial growth of Au nanoparticles on the CuS, which demonstrates superior peroxidase (POD)-mimic and SERS activity. Mechanistic studies indicate that plasmon-enhanced catalytic and SERS activity can be attributed to the spatially separated CuS-Au heterostructure, which supports the light-triggered hot electron-hole pairs production and localized surface plasmon resonance hotspots. For practical biosensing, the CuS-Au heterostructures assembled lateral flow assay (LFA) was used for SERS/catalytic colorimetric/photothermal three-mode detection of Streptococcus pneumoniae and Klebsiella pneumoniae, with visually colorimetric mode at 103 CFU/mL and quantitative SERS/photothermal modes at 2-10² CFU/mL within 15 min, 15 clinical samples were used to validate the assay, the result was 100% concordant to the results of quantitative real-time PCR. This study provides a unique avenue to controllably produce plasmon-enhanced nanozyme, which can provide multi-mode signals for LFA application and meet the requirements of different scenarios.