Fatty acid metabolism in colon cancer patients

This study was conducted on patients with colon cancer. The study included 60 people with colon cancer (men and women) and 60 healthy people without any apparent disease. The patients were diagnosed by specialized doctors and the collection period was between September to December 2022. Blood serum was isolated from blood samples collected from both patients and healthy people (the latter being the control group) and then divided into several parts. Several biochemical variables were also measured in the blood serum in the second part, where the levels of total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) were measured via the enzymatic method and using a dry kit (Kit). Patients with colon cancer had higher levels of low- d ensity lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C) compared to the control . For the third part of the blood serum, the serum lipids were extracted using organic solvents and separated from each other using thin layer chr o matography (TLC). The percentage of saturated and polyunsaturated fatty acids (mono and poly) was determined via gas chromat o graphy (CGC) for the three fractions of blood serum (CE, TG, and PL) after fatty acid re-esterification. It was observed that there we re differences between the percentages of fatty acids of various types in the patient and the control groups, and in the different parts of the blood serum lipids; this indicates the effect of fatty acids in the development of colon cancer, including risk factors for card i ovascular disease through the differences and imbalance that occurs in the percentage, especially for polyunsaturated fatty acids (PUFA).