DEVELOPMENT AND OPTIMIZATION OF A MULTIFUNCTIONAL BIOSENSOR FOR DETERMINATION OF PYRUVATE CONCENTRATION AND/OR ALT

In this work, an amperometric biosensor for highly sensitive and selective determination of pyruvate concentration was developed and optimized and the feasibility of it’s use for the analysis of alanine aminotransferase (ALT) activity was demonstrated. A platinum disk electrode was used as a transducer, and the bioselective element was based on pyruvate oxidase and PVA-SBQ photopolymer. The influence of solution parameters (buffer capacity, pH, ionic strength) was analyzed, the selectivity of the biosensor towards interferents and the reproducibility of responses (RSD = 12.66%) were studied. The main analytical characteristics of the biosensor were also analyzed (linear range of 10–500 μM pyruvate, sensitivity 66 nA/mM, limit of detection 1.57 μM). The obtained data indicate the prospects of using the developed biosensor for the determination of pyruvate in biological samples. Finally, a calibration curve of the response of the developed pyruvate-sensitive biosensor on the ALT content was created.