Biomanufacturing and lipidomics analysis of extracellular vesicles secreted by human blood vessel organoids in a vertical wheel bioreactor
Human BVOs differentiated in VWBRs (in particular 40 rpm) produce 2-3 fold higher yield of EVs (per mL) than static control. The bio manufactured EVs from VWBRs have exosomal characteristics and therapeutic cargo, showing functional properties in in vitro assays. This innovative approach establishes VWBRs as a scalable platform for producing functional EVs with defined lipid profiles and therapeutic potential, paving the way for future in vivo studies.
Authors
- Sunghoon Jung (ORCID: https://orcid.org/0000-0003-3183-5249)
- Chang Liu (ORCID: https://orcid.org/0000-0002-2749-7542)
- Changchun Zeng (ORCID: https://orcid.org/0000-0003-0855-3497)
- Zixiang Liu (ORCID: https://orcid.org/0000-0001-7487-450X)
- Li Sun (ORCID: https://orcid.org/0000-0001-8228-7487)
- Pradeepraj Durairaj (ORCID: https://orcid.org/0000-0002-6312-6589)
- D. Berry (ORCID: https://orcid.org/0000-0003-2672-0826)
- Justice Ene (ORCID: https://orcid.org/0009-0004-4723-7299)
- Yan Li (ORCID: https://orcid.org/0000-0002-5938-8519)
- Farhat Syed
Institutions
- Florida State University (US)
- Florida A&M University - Florida State University College of Engineering (US)
- 490 BioTech (United States) (US)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-04-24
- DOI
- https://doi.org/10.1186/s13287-025-04317-2
- Citations
- 10
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 8.06
Funders
- National Science Foundation
- Foundation for the National Institutes of Health
- Florida State University
- National Institutes of Health
- Directorate for Biological Sciences
- Division of Chemical, Bioengineering, Environmental, and Transport Systems