IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunity
Identification of isocitrate dehydrogenase (IDH) mutations has uncovered the crucial role of metabolism in gliomagenesis. Oncolytic herpes virus (oHSV) initiates direct tumor debulking by tumor lysis and activates anti-tumor immunity, however, little is known about the role of glioma metabolism in determining oHSV efficacy. Here we identify that oHSV rewires central carbon metabolism increasing glucose utilization towards oxidative phosphorylation and shuttling glutamine towards reductive carboxylation in IDH wildtype glioma. The switch in metabolism results in increased lipid synthesis and cellular ROS. PKC induces ACSL4 in oHSV treated cells leading to lipid peroxidation and ferroptosis. Ferroptosis is critical to launch an anti-tumor immune response which is important for viral efficacy. Mutant IDH (IDHR132H) gliomas are incapable of reductive carboxylation and hence ferroptosis. Pharmacological blockade of IDHR132H induces ferroptosis and anti-tumor immunity. This study provides a rationale to use an IDHR132H inhibitor to treat high grade IDH-mutant glioma patients undergoing oHSV treatment.
Authors
- Tae Jin Lee (ORCID: https://orcid.org/0000-0002-5245-1140)
- Ashok Sharma (ORCID: https://orcid.org/0000-0001-9597-4374)
- Benny Abraham Kaipparettu (ORCID: https://orcid.org/0000-0003-3444-4307)
- Nagireddy Putluri (ORCID: https://orcid.org/0000-0003-4488-7400)
- Alexander Ling (ORCID: https://orcid.org/0000-0001-6194-9875)
- Vasanta Putluri (ORCID: https://orcid.org/0000-0001-7943-2209)
- Joshua C. Anderson (ORCID: https://orcid.org/0000-0002-6977-9054)
- Sara A. Murphy (ORCID: https://orcid.org/0000-0003-2722-0081)
- E. Antonio Chiocca (ORCID: https://orcid.org/0000-0001-5183-1670)
- Pamela L. Wenzel (ORCID: https://orcid.org/0000-0002-4530-015X)
- Balveen Kaur (ORCID: https://orcid.org/0000-0001-7738-0804)
- Upasana Sahu (ORCID: https://orcid.org/0000-0003-2473-9312)
- James M. Markert (ORCID: https://orcid.org/0000-0001-7457-7329)
- Abu Hena Mostafa Kamal (ORCID: https://orcid.org/0000-0001-6750-0729)
- Christopher D. Willey (ORCID: https://orcid.org/0000-0001-9953-0279)
- Jun Hyoung Park
- Matthew P. Mullarkey
Institutions
- Brigham and Women's Hospital (US)
- The University of Texas MD Anderson Cancer Center (US)
- Baylor College of Medicine (US)
- Augusta University (US)
- University of Alabama at Birmingham (US)
- Augusta University Health (US)
- Children's Cancer Center (US)
- The University of Texas Health Science Center at Houston (US)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-04-24
- DOI
- https://doi.org/10.1038/s41467-025-58911-2
- Citations
- 10
- Primary Topic
- Cancer, Hypoxia, and Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 9.36
Funders
- Cancer Prevention and Research Institute of Texas
- Emory University
- Dan L. Duncan Cancer Center, Baylor College of Medicine
- Augusta University
- National Institutes of Health
- National Cancer Institute
- National Institute of Neurological Disorders and Stroke