Serum Metabolomic Profiling in Healthy Dogs Supplemented with Increasing Levels of Purified Beta-1,3/1,6-Glucans

Metabolomics has proven to be an effective tool for elucidating mechanisms and assessing the effectiveness of dietary interventions in canine and feline nutrition. In this context, the present study aimed to perform a metabolomic analysis of the serum of dogs supplemented with increasing levels of beta-1,3/1,6-glucans to generate evidence and gain a deeper understanding of the metabolic responses associated with this supplementation. Eight dogs were evenly assigned to two balanced 4 × 4 Latin squares. Four diets were tested, differing only in beta-glucan content (0.0%, 0.07%, 0.14%, and 0.28%), and the dogs were fed according to their individual maintenance energy requirements. Each experimental period lasted 35 days. On day 35, 5 mL of blood was collected via jugular venipuncture to obtain serum for metabolomic analysis. Nuclear magnetic resonance analysis identified 12 key serum metabolites. Hierarchical heat map analysis revealed differences in metabolite intensity between treatments (<i>p</i> < 0.05). Additionally, the most relevant metabolic pathways were phenylalanine, tyrosine, and tryptophan metabolism; alanine, aspartate, and glutamate metabolism; and glyoxylate and dicarboxylate metabolism. This study demonstrated that increasing levels of purified beta-1,3/1,6-glucans from <i>Saccharomyces cerevisiae</i> modulated key metabolic pathways in dogs, particularly those related to amino acid, lipid and energy metabolisms, and gut microbiota. These findings provide insights into the mechanisms by which beta-glucans influence canine health.