New scientific evidence for the history and occupants of Tomb I (“Tomb of Persephone”) in the Great Tumulus at Vergina

The Great Tumulus of Vergina (Aegae) is considered to be the royal burial complex of the Macedonian kings. Beneath it four tombs were discovered, labeled Tomb I, II, III and IV. Several hypotheses have been proposed for the identities of the occupants of the “royal tombs”, but without scientific backing. We present new data from Tomb I (“The Tomb of Persephone”), which contained inhumed (unburnt), in situ and commingled adult skeletal remains, as well as commingled nonadult and animal bones. We applied a range of scientific techniques, including radiocarbon dating, ancient DNA (aDNA), strontium and stable carbon and nitrogen isotope analysis, supported by osteological and odontological observations on the adult and nonadult bones found in Tomb I to provide concrete evidence for the date of burials, sex, age at death and origin of the individuals interred in this tomb. Our results show that, with the exception of four bones that were identified as female, all the adult bones are male according to the aDNA and osteological results, and they belonged to a man aged 25–35 years with a stature of approximately 167 cm. Radiocarbon dating places this burial in the first half of the 4th century calBC, specifically between 400 and 367 calBC, and by applying a potential collagen offset correction this is slightly shifted to 388-356 calBC at the latest. The female bones date to the same period. However, all the nonadults and animal bones, date to the Roman period from 150 calBC, the earliest to 130 calAD the latest. Therefore, these are not related to the primary adult burials. The male occupant was most likely an important Macedonian royal of the Argead/Temenid house who died in the period 388-356 calBC and was probably honored or worshipped in the shrine above and entombed likely together with a female. Previous suggestions that the skeletal remains belong to Philip II, his wife Cleopatra and newborn child are not scientifically sustainable. • The results reveal a male burial, aged 25–35, in the first half of the 4th century BC. • Strontium isotopes showed this male spent his childhood away from Vergina/Pella. • Few female bones point also to a female burial, aged 18–25 years, in the same time. • Infant bones date to Roman period indicating access of the tomb at much later times. • The royal individuals interred in this tomb decades before Philip's II death.