PDGF-BB overexpressing dental pulp stem cells improve angiogenesis in dental pulp regeneration
The CD73 (+) PDGF (+) subpopulation demonstrated spatial correlation with CD31 (+) vasculature. PDGF-BB overexpression enhanced DPSCs' proliferative capacity and migration capacity. Co-cultured HUVECs exhibited increased tube formation with PDGF-BB group. <i>In vivo</i> transplants generated more vascular structures containing CD31 (+) endothelia. These findings establish PDGF-BB engineering as an effective strategy to amplify DPSCs' angiogenic potential, while emphasizing the therapeutic value of functionally-defined stem cell subpopulations in pulp regeneration.
Authors
- Yishuai Li
- Huijiao Yan (ORCID: https://orcid.org/0000-0001-6406-9005)
- Shensheng Gu (ORCID: https://orcid.org/0000-0001-7444-4905)
- Wentao Jiang (ORCID: https://orcid.org/0000-0002-2064-6760)
- Chenli Si
- Wenjie Zhang (ORCID: https://orcid.org/0000-0002-7753-695X)
- Weiping Li (ORCID: https://orcid.org/0000-0002-1263-3791)
- Ningwei Xu
- Shuhan Duan
Institutions
- Shanghai Jiao Tong University (CN)
- National Clinical Research Center for Digestive Diseases (CN)
- Shanghai Stomatological Hospital (CN)
- Shanghai Ninth People's Hospital (CN)
- Nanjing Medical University (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-04-24
- DOI
- https://doi.org/10.3389/fbioe.2025.1578410
- Citations
- 4
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 6.50