Development and validation of HPLC method for determination of amino acid mole ratio in Semaglutide

This study describes the development and validation of HPLC method for determination of amino acids mole ratio in Semaglutide. System suitability, specificity, Linearity and Range, Precision at specification level and Solution stability, Accuracy were studied to ensure amino acid mole ratio. The analytical method used for determination of amino acid mole ratio. System Suitability, Specificity, Linearity and range, Precision at specification level, and Accuracy. Hence, the method stands validated. The method can be used routinely for determination of amino acid mole ratio in Semaglutide in the quality control department.