Selection and Identification of Two Electrogenic Bacteria and Their Performance in Wastewater Treatment

The potential applications of microbial fuel cells (MFCs) in wastewater treatment and energy recovery have been investigated. MFCs harness the metabolic activity of microorganisms to generate electricity from organic compounds, which is highly important for preventing environmental pollution and constructing an ecological civilization. To address the safety limitations of pathogenic electrogenic bacteria in MFCs, this study isolated two novel nonpathogenic strains, Cellulosimicrobium 99-1 and Listeria innocua 5-2, from livestock samples. Their synergistic interaction boosted voltage output by 8.2% (exceeding 400 mV) compared to pure cultures while achieving 90.6% chemical oxygen demand (COD) and 74% total nitrogen removal in domestic wastewater, demonstrating dual advantages in biosecurity and treatment efficiency. This study provides experimental data and theoretical support for the application of MFC technology in environmental engineering, laying a foundation for further optimizing MFC design and improving wastewater treatment efficiency.