Continuous collection of human mesenchymal-stromal-cell-derived extracellular vesicles from a stirred tank reactor operated under xenogeneic-free conditions for therapeutic applications
Overall, the scalable and Good Manufacturing Practices (GMP)-compliant platform established herein enabled the reproducible manufacturing of MSC-EVs with high purity and generally accepted characteristics concerning size, protein markers, surface charge, morphology, and cellular internalization, validating its potential for future clinical applications.
Authors
- Joaquim M. S. Cabral (ORCID: https://orcid.org/0000-0002-2405-5845)
- William O.S. Salvador (ORCID: https://orcid.org/0000-0002-5539-3433)
- Ana Fernandes‐Platzgummer (ORCID: https://orcid.org/0000-0002-5367-6528)
- Cristiana Ulpiano (ORCID: https://orcid.org/0000-0002-3442-793X)
- Yee-Hsien Lin
- Teresa Franchi-Mendes (ORCID: https://orcid.org/0000-0003-2297-5274)
- Cláudia L. da Silva (ORCID: https://orcid.org/0000-0002-1091-7651)
- Carlos A. V. Rodrigues (ORCID: https://orcid.org/0000-0001-9645-1591)
- Gabriel A. Monteiro (ORCID: https://orcid.org/0000-0003-4840-6299)
- Min-Chang Huang
- Han-Tse Lin
Institutions
- University of Lisbon (PT)
- Universidade Politécnica de Lisboa (PT)
- Flow International Corporation (United States) (US)
- Institute for Biotechnology and Bioengineering (PT)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-04-24
- DOI
- https://doi.org/10.1186/s13287-025-04341-2
- Citations
- 18
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 14.51
Funders
- Institute for Bioengineering and Biosciences
- Fundação para a Ciência e a Tecnologia