Uncovering the Benefits of Epicatechin for Oxidative Stress in Human Health

Objective: Epicatechin (EC) is one of the major components of green tea catechins. This study investigated the effect of the amount of epicatechin obtained by brewing green tea under optimal conditions against oxidative stress induced by hydrogen peroxide (H2O2). Materials and Methods: In peripheral blood mononuclear cells (PBMCs), the amount of epicatechin determined by brewing green tea under optimum conditions was applied against 250 µM H2O2 and cell viability was determined by MTT test, total antioxidant status (TAS) and total oxidant status (TOS) were determined by biochemical analysis, apoptosis-related Bax, Bcl2, p53 expression analysis was determined by qRT-PCR method. Results: The cell viability was significantly higher in the H2O2+EC group than in the H2O2 group (p