MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines
Building on the collaborative efforts of an international team of researchers, we present updates, simplifications, and new recommendations to the original MIQE guidelines, designed to maintain their relevance and applicability in the context of emerging technologies and evolving qPCR applications.
Authors
- Reinhold Mueller
- Malcolm Burns (ORCID: https://orcid.org/0000-0001-7585-6741)
- Maurice J.B. van den Hoff (ORCID: https://orcid.org/0000-0002-3837-8055)
- Michael W. Pfaffl (ORCID: https://orcid.org/0000-0002-3192-1019)
- Stephen A. Bustin (ORCID: https://orcid.org/0000-0003-1870-6098)
- Andreas Untergasser
- Mikael Kubista (ORCID: https://orcid.org/0000-0002-2940-352X)
- Carl T. Wittwer (ORCID: https://orcid.org/0000-0002-9993-8263)
- Jim F. Huggett (ORCID: https://orcid.org/0000-0002-0945-1911)
- Mojca Milavec (ORCID: https://orcid.org/0000-0002-5794-2109)
- Stefan Rödiger (ORCID: https://orcid.org/0000-0002-1441-6512)
- Nham Tran (ORCID: https://orcid.org/0000-0001-7747-2530)
- Jo Vandesompele (ORCID: https://orcid.org/0000-0001-6274-0184)
- Jan M. Ruijter
- Tania Nolan
- Gregory L. Shipley
Institutions
- University of Technology Sydney (AU)
- National Physical Laboratory (GB)
- Czech Academy of Sciences (CZ)
- Heidelberg University (DE)
- Ghent University (BE)
- Czech Academy of Sciences, Institute of Biotechnology (CZ)
- National Measurement Laboratory (AU)
- National Institute of Biology (SI)
- Amsterdam University Medical Centers (NL)
- Anglia Ruskin University (GB)
- Brandenburg University of Technology Cottbus-Senftenberg (DE)
- Technical University of Munich (DE)
Publication Details
- Journal
- Clinical Chemistry
- Published
- 2025-04-24
- DOI
- https://doi.org/10.1093/clinchem/hvaf043
- Citations
- 100
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 80.60