DETERMINATION OF CASEOUS NECROSIS TO LYMPHOCYTE DENSITY IN HISTOPATHOLOGICAL RESPONSE OF HOST IMMUNE SYSTEM AGAINST MYCOBACTERIAL PATHOGENS

In mycobacterium infection, the characteristics of necrosis and inflammatory cells vary among host species and its hallmark in the diagnosis of a particular infectious disease remains unclear. Therefore, this study aimed to identify and characterize the morphological features of caseous necrosis with lymphocytes against mycobacterial pathogens in humans and animals. During this study, a total of 126 extrapulmonary tissue samples, and demographic data of individuals and animals suspected of mycobacterium infection in the Faisalabad district were collected and processed further at Al-Shifa Diagnostic and Research Laboratory, Faisalabad. In the results, the frequency analysis (%) showed that there were 21, 46, 38, and 21 Mycobacterium (MB) positive individuals in the 20-30, 31-40, 41-50, and 51-60 age groups. The maximum number of MB-positive individuals were in the 31-40 age group with a maximum number of females and males were more in the 41-50 age group. The presence of caseation necrosis in 110 (88%) individuals. The examination of the hematoxylin and eosin-stained slides revealed the presence of caseation in 22 (88%) and 3 (12%) showed no caseation. It is concluded that the host immune response is manifested with the development of caseation necrosis with lymphocyte density and is an applicable comprehensive approach to comparing histopathological markers in both animals and humans.