Successful prediction of LC8 binding to intrinsically disordered proteins sheds light on AlphaFold’s black box

Correlations between binding affinities and AlphaFold scores provide insight into the black box and indicate that AlphaFold learned an inaccurate energy function that nevertheless is useful for making inferences and conclusions about physical systems. Binding sites predicted by this method can be prioritized for investigation by comparing to result by LC8Pred, local structure, and evolutionary conservation.