Analytical assessment of Sukumara Ghrita using HPTLC and GCMS: Exploring its phytoconstituents and probable mode of action

Background: Sukumara Ghrita an Ayurvedic ghee-based formulation is renowned for its efficacy in addressing various ailments. Objective: This study employs advanced analytical techniques, specifically High-Performance Thin-Layer Chromatography (HPTLC) and Gas Chromatography-Mass Spectrometry (GC-MS), to comprehensively characterize Sukumara Ghrita and to explore bioactive principles present in Sukumara Ghrita and understand their probable mode of action in treating various ailments. Materials and Methods: The medicine was procured from GMP certified Sitaram Ayurveda Pharma and was subjected to HPTLC and GC–MS analysis after due processing. The methanolic extracts were used for HPTLC and GCMS studies. HPTLC fingerprints for methanolic extracts of Sukumara Ghrita was established with good separation and resolution in solvent system Toluene: Ethyl acetate: Hexane (6:3:1). Results: GC-MS analysis of Sukumara Ghrita revealed the presence of 11 phytochemical compounds. HPTLC analysis detected the presence of 9 and 14 active biological constituents at 254 nm and 366 nm respectively, which remains to be identified. Conclusion: The probable mode of action of Sukumara ghrita can be understood through the action of phytoconstituents screened through HPTLC and GC-MS. The phytoconstituents screened are said to have Anti-oxidant, Anti-hypercholesterolemic, Antihypertriglyceridemic, Anti-inflammatory etc actions.