Isolation of Effective Wound Healing Compounds from Chloroform Fraction of Ethanol Extract of Ehretia Laevis Roxb.(KhanduChakka/AjanVruksha)

Phytochemical analysis plays a very important role in identifying effective components of herbs. Ehretia Laveis Roxb. herb not much known to world identified for study having very good capacity of wound healing and pain relief. Ehretia laevis Roxb.is a rare species from the boraginaceae family. For phytochemical analysis, ethanol extractions of Ehtretia Laevis Roxb. leaves were carried out using the soxhlet extraction method. Fractionation of collected ethanol extract was carried out using n-hexane, methanol, chloroform and water as a solvent with the help of a separating funnel. Wound healing action of n-hexane, chloroform, methanol and water extracts were carried out. It was observed that chloroform fraction was effective for wound healing in animal models and hence chloroform fraction of ethanol extract of Ehretia Laevis Roxb. was taken for further phytochemical analysis. Column chromatography of chloroform fraction was carried out using silica gel. 5 Fractions were isolated by using Column Chromatography. Fraction -2 was in sufficient quantity and hence taken for wound healing study. Fraction -2- Dragendorff’s test- positive for alkaloids. The rats were marked and divided them into 6 groups of 6 animals each. Isolated fraction-2-2%w/w treated group wound healing was 77.75±0.312 %. The Povidone iodineclosesthe wound by 96.63±0.205% which is close to the group appliedby 4% w/w ointments of isolated fraction-2 i.e. 94.10±0.146 %. Fraction -2- was characterized for1H NMR, 13CNMR and LCMS. On comparing experimental data of fraction-2 , it is observed that the experimental data match with literature data of α amyrin and β amyrin. From this, we can conclude that the isolated fraction-2 is the mixture of α amyrin and β amyrin responsible for wound healing. From the above study, we can conclude that mixture of α amyrin and β amyrin may have wound healing prosperities.