Multidimensional multiplexing metalens for STED microscopy

Stimulated emission depletion (STED) microscopy is a versatile super-resolution imaging technique for life sciences and data storage. Despite continuous breakthroughs, modern STED microscopes are still relatively bulky and limited to laboratory setups. Here, we exploit the multidimensional multiplexing properties of metalenses and experimentally demonstrate the realization of a compact STED lens with a single metasurface. A 635-nm right-handed circularly polarized excitation laser is focused by the metalens into a diffraction-limited Gaussian beam, while a 780-nm depletion beam with opposite chirality is converted into a high-quality donut-shaped focus on the same plane. As a consequence, STED super-resolution imaging based on the metalens has been obtained by recording the unpolarized photoluminescence using the same metalens. The experimentally demonstrated resolution reaches 0.7× of the diffraction limit and can be further improved. This study represents a critical step toward the miniaturization and integration of STED microscope.

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Publication Details

Journal
Science Advances
Published
2025-04-23
DOI
https://doi.org/10.1126/sciadv.adt2807
Citations
11
Primary Topic
Metamaterials and Metasurfaces Applications
Type
article
Field-Weighted Citation Impact
4.70
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article

Multidimensional multiplexing metalens for STED microscopy

Xing Ma, Zhengtong Liu, Qinghai Song, Shumin Xiao et al.
11 citations
Science Advances
Metamaterials and Metasurfaces Applications
4.70
article

Multidimensional multiplexing metalens for STED microscopy

Xing Ma, Zhengtong Liu, Qinghai Song, Shumin Xiao, Ziheng Ji, Xinbo Sha, Haili Wang, Qinmiao Chen
article en
11 citations

Abstract

Stimulated emission depletion (STED) microscopy is a versatile super-resolution imaging technique for life sciences and data storage. Despite continuous breakthroughs, modern STED microscopes are still relatively bulky and limited to laboratory setups. Here, we exploit the multidimensional multiplexing properties of metalenses and experimentally demonstrate the realization of a compact STED lens with a single metasurface. A 635-nm right-handed circularly polarized excitation laser is focused by the metalens into a diffraction-limited Gaussian beam, while a 780-nm depletion beam with opposite chirality is converted into a high-quality donut-shaped focus on the same plane. As a consequence, STED super-resolution imaging based on the metalens has been obtained by recording the unpolarized photoluminescence using the same metalens. The experimentally demonstrated resolution reaches 0.7× of the diffraction limit and can be further improved. This study represents a critical step toward the miniaturization and integration of STED microscope.

Science AdvancesVol. 11(17)
Shanxi University (CN), Shenzhen Bay Laboratory (CN), Peng Cheng Laboratory (CN), Shenzhen Technology University (CN), Ministry of Industry and Information Technology (CN)
Responsible consumption and production
Openalex Percentile: Top 5%
Metamaterials and Metasurfaces Applications
4.70
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Multidimensional multiplexing metalens for STED microscopy — Xing Ma, Zhengtong Liu, et al. · Science Advances (2025) | TGRS Research Map | TGRS