Evaluation of single-dose inhalation of clarithromycin-loaded solid lipid nanoparticles in rat model

This study aimed to explore the effective utilization of clarithromycin (CLA) through the development of clarithromycin-loaded solid lipid nanoparticles (CLA-loaded SLN), designed for nebulizer-based delivery for the first time. Wistar albino rats were divided into seven groups (n=8) based on time points (0.5, 1, 2, 4, 12, 24 hours, and a control group). At the respective time points, lung tissues and blood samples were collected and analyzed for CLA concentrations using HPLC-MS/MS. The maximum serum concentration (Cmax) was 6.74 µg/mL, with average serum CLA concentrations of 5.06, 2.5, 2.18, 1.13, and 0.5 µg/mL across the groups. CLA was undetectable in the control group and in the serum of the last group. Using the linear trapezoidal method (LTM), the area under the curve (AUC 0-24) for serum CLA was calculated as 17.06 µg*h/mL. Significant differences (p