Comparative in vitro study of chondrocyte viability and gene expression in wrought and additive manufactured CoCrMo sliding against articular cartilage

Hemiarthroplasty consists of the replacement of local cartilage defects by a partial implant and provides a less aggressive alternative to total joint replacement. The material frequently selected nowadays for partial implants is CoCrMo alloy, which results in a sliding contact between articular cartilage and the alloy. Since the geometry of the implant needs to be tailored to the patient, partial implant technology would greatly profit from novel additive manufacturing techniques. This study examines the feasibility of using additive manufacturing techniques on partial implants made of CoCrMo alloys, with a particular focus on the impact of manufacturing technique on mechanical stimulation, cartilage analysis, and friction performance. To this end, in vitro biotribological experiments were performed between CoCrMo samples and bovine articular cartilage using PBS as simulated body fluid. Key findings reveal significant changes in microstructure between laser beam melted (LBM) and wrought CoCrMo alloys, despite having a comparable elemental composition. The coefficient of friction (CoF) measured between bovine articular cartilage and the CoCrMo specimens during biotribocorrosive testing revealed no significant differences resulting from the manufacturing techniques, even though wrought CoCrMo resulted in a higher reproducibility. Conventionally produced CoCrMo also exhibited a more anodic open circuit potential during the experiments, likely due to the significant differences in microstructure that affect corrosion resistance. The tested cartilage samples showed a slight increase in MMP13 (Matrix Metalloproteinases - degradative enzymes) in comparison to the controls, indicating potential remodeling effects, especially for the LBM CoCrMo alloy. Additionally, the metabolic activity in the cartilage specimens increased due to mechanical stimulation. No cracks or fissures were detected in histological imaging thus highlighting that the cartilage samples were not damaged during harvesting or testing. These findings indicate the possibility of an equivalent use of additive manufactured CoCrMo, enabling patient-specific surgeries and encourage further research to explore the long-term impact of corrosion stability on implant longevity and functionality.