Scalable Hyperpolarized MRI Enabled by Ace‐SABRE of [1‐ <sup>13</sup> C]Pyruvate
Abstract Hyperpolarized (HP) MRI using [1– 13 C]pyruvate is emerging as a promising molecular imaging approach. Among hyperpolarization methods, Signal Amplification By Reversible Exchange (SABRE) is attractive because SABRE polarizes the substrates directly in room‐temperature solutions avoiding complex hardware. Most SABRE experiments have historically been performed in methanol, a relatively toxic and difficult‐to‐remove solvent. Here we demonstrate the use of a 80/20 acetone/water (A/W) solvent system (Ace‐SABRE) to provide hyperpolarized [1– 13 C]pyruvate with up to 17% polarization, then implement a solvent processing protocol to achieve injectable solutions retaining 74% of the initial polarization, and lastly we demonstrate HP in vivo spectroscopy and imaging using the Ace‐SABRE platform to showcase metabolic tracking in a hepatocellular carcinoma (HCC) tumor as well as HP‐MRI, both in direct comparison to dissolution dynamic nuclear polarization (d‐DNP) experiments. The Ace‐SABRE technique promises faster adoption of SABRE hyperpolarization in biological experiments, overall lowering the barriers to entry for HP‐NMR and HP‐MRI.
Authors
- Stephen Kadlecek (ORCID: https://orcid.org/0000-0002-5131-7994)
- Ishani M. Senanayake (ORCID: https://orcid.org/0009-0003-4772-2983)
- Boyd M. Goodson (ORCID: https://orcid.org/0000-0001-6079-5077)
- Thomas Theis (ORCID: https://orcid.org/0000-0001-6779-9978)
- Thomas Boele (ORCID: https://orcid.org/0000-0001-9322-7762)
- Bukola Adebesin
- Simon B. Duckett (ORCID: https://orcid.org/0000-0002-9788-6615)
- Carlos Dedesma
- Alexander I. Zavriyev (ORCID: https://orcid.org/0000-0003-3383-0724)
- Erica Curran (ORCID: https://orcid.org/0009-0005-1376-5541)
- Megan Pike (ORCID: https://orcid.org/0000-0002-2896-5408)
- Patrick TomHon (ORCID: https://orcid.org/0000-0003-3202-9812)
- Jared M. Harzan
- Mustapha Abdulmojeed (ORCID: https://orcid.org/0000-0002-5513-3658)
- Hong Yuan (ORCID: https://orcid.org/0000-0002-0535-3214)
- Franziska Theiß (ORCID: https://orcid.org/0009-0002-3273-8950)
- Eduard Y. Chekmenev (ORCID: https://orcid.org/0000-0002-8745-8801)
- T. Gade (ORCID: https://orcid.org/0000-0001-8783-0661)
- Sheng Shen (ORCID: https://orcid.org/0000-0002-4320-2732)
- Matthew S. Rosen (ORCID: https://orcid.org/0000-0002-7194-002X)
- Luke Tucker
- Stephen McBride (ORCID: https://orcid.org/0000-0001-9144-1423)
Institutions
- Southern Illinois University Carbondale (US)
- University of North Carolina at Chapel Hill (US)
- The University of Sydney (AU)
- North Carolina State University (US)
- Wayne State University (US)
- Massachusetts General Hospital (US)
- The Barbara Ann Karmanos Cancer Institute (US)
- Enzo Life Sciences (United States) (US)
- Athinoula A. Martinos Center for Biomedical Imaging (US)
- University of York (GB)
- University of Pennsylvania (US)
Publication Details
- Journal
- Angewandte Chemie
- Published
- 2025-04-23
- DOI
- https://doi.org/10.1002/ange.202501231
- Citations
- 2
- Primary Topic
- Advanced NMR Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.51
Funders
- National Science Foundation
- National Institutes of Health