Scalable Hyperpolarized MRI Enabled by Ace‐SABRE of [1‐ <sup>13</sup> C]Pyruvate
Hyperpolarized (HP) MRI using [1-<sup>13</sup>C]pyruvate is emerging as a promising molecular imaging approach. Among hyperpolarization methods, Signal Amplification By Reversible Exchange (SABRE) is attractive because SABRE polarizes the substrates directly in room-temperature solutions avoiding complex hardware. Most SABRE experiments have historically been performed in methanol, a relatively toxic and difficult-to-remove solvent. Here we demonstrate the use of a 80/20 acetone/water (A/W) solvent system (Ace-SABRE) to provide hyperpolarized [1-<sup>13</sup>C]pyruvate with up to 17% polarization, then implement a solvent processing protocol to achieve injectable solutions retaining 74% of the initial polarization, and lastly we demonstrate HP in vivo spectroscopy and imaging using the Ace-SABRE platform to showcase metabolic tracking in a hepatocellular carcinoma (HCC) tumor as well as HP-MRI, both in direct comparison to dissolution dynamic nuclear polarization (d-DNP) experiments. The Ace-SABRE technique promises faster adoption of SABRE hyperpolarization in biological experiments, overall lowering the barriers to entry for HP-NMR and HP-MRI.
Authors
- Stephen Kadlecek (ORCID: https://orcid.org/0000-0002-5131-7994)
- Ishani M. Senanayake (ORCID: https://orcid.org/0009-0003-4772-2983)
- Boyd M. Goodson (ORCID: https://orcid.org/0000-0001-6079-5077)
- Thomas Theis (ORCID: https://orcid.org/0000-0001-6779-9978)
- Thomas Boele (ORCID: https://orcid.org/0000-0001-9322-7762)
- Bukola Adebesin
- Simon B. Duckett (ORCID: https://orcid.org/0000-0002-9788-6615)
- Carlos Dedesma
- Alexander I. Zavriyev (ORCID: https://orcid.org/0000-0003-3383-0724)
- Erica Curran (ORCID: https://orcid.org/0009-0005-1376-5541)
- Megan Pike (ORCID: https://orcid.org/0000-0002-2896-5408)
- Patrick TomHon (ORCID: https://orcid.org/0000-0003-3202-9812)
- Jared M. Harzan
- Mustapha Abdulmojeed (ORCID: https://orcid.org/0000-0002-5513-3658)
- Hong Yuan (ORCID: https://orcid.org/0000-0002-0535-3214)
- Franziska Theiß (ORCID: https://orcid.org/0009-0002-3273-8950)
- Eduard Y. Chekmenev (ORCID: https://orcid.org/0000-0002-8745-8801)
- T. Gade (ORCID: https://orcid.org/0000-0001-8783-0661)
- Sheng Shen (ORCID: https://orcid.org/0000-0002-4320-2732)
- Matthew S. Rosen (ORCID: https://orcid.org/0000-0002-7194-002X)
- Luke Tucker
- Stephen McBride (ORCID: https://orcid.org/0000-0001-9144-1423)
Institutions
- Southern Illinois University Carbondale (US)
- University of North Carolina at Chapel Hill (US)
- The University of Sydney (AU)
- North Carolina State University (US)
- Wayne State University (US)
- Massachusetts General Hospital (US)
- The Barbara Ann Karmanos Cancer Institute (US)
- Enzo Life Sciences (United States) (US)
- Athinoula A. Martinos Center for Biomedical Imaging (US)
- University of York (GB)
- University of Pennsylvania (US)
Publication Details
- Journal
- Angewandte Chemie International Edition
- Published
- 2025-04-23
- DOI
- https://doi.org/10.1002/anie.202501231
- Citations
- 11
- Primary Topic
- Advanced NMR Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 8.32
Funders
- National Science Foundation
- U.S. Department of Energy
- North Carolina State University
- Government of the United Kingdom
- UK Research and Innovation
- Kinghorn Foundation
- National Institutes of Health
- HORIZON EUROPE Framework Programme
- National Health and Medical Research Council
- Division of Chemistry
- National Center for Advancing Translational Sciences
- Biological and Environmental Research
- North Carolina Translational and Clinical Sciences Institute, University of North Carolina at Chapel Hill